anti tbk1 3504 Search Results


risedronate  (Bio-Techne corporation)
99
Bio-Techne corporation risedronate
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anti tbk1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti tbk1
Anti Tbk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total tbk1 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc total tbk1 antibody
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anti tbk  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti tbk
Anti Tbk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tbk1  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti tbk1
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anti tbk1  (Proteintech)
97
Proteintech anti tbk1
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
Anti Tbk1, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti tbk1 nak  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti tbk1 nak
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
Anti Tbk1 Nak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tbk1/nak antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc tbk1/nak antibody
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
Tbk1/Nak Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3993  (Bio-Techne corporation)
99
Bio-Techne corporation 3993
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
3993, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-sting 13647  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-sting 13647
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
Anti Sting 13647, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antiirf3 4302  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc antiirf3 4302
A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding <t>TBK1,</t> NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.
Antiirf3 4302, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding TBK1, NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.

Journal: bioRxiv

Article Title: RTN3 inhibits RIGI-I-mediated antiviral responses by impairing TRIM25-mediated K63-linked polyubiquitination

doi: 10.1101/2020.12.01.407320

Figure Lengend Snippet: A. Luciferase assays of HEK293T cells transfected with an ISRE luciferase reporter (ISRE-Luc), an IFNB luciferase reporter (IFNβ-Luc) or an NF-κB luciferase reporter (NF-κB-Luc) together with an HA-tagged empty vector (HA-Ev, no wedge) or increasing amounts (wedge) of plasmid encoding RTN3 (HA-RTN3) followed by transfection with a plasmid encoding the RIG-I CARD domain [RIG-I (N)] to activate the pathway. Cell lysates were collected 24 h post transfection, the same as that described in the following experiments, if no additional annotation was performed. B. Luciferase activity of HEK293T cells transfected with ISRE-Luc, together with HA-Ev or increasing amounts of plasmid HA-RTN3, followed by treatment with poly(I:C) (5 μg/ml) or SeV (MOI = 0.1) for 16 or 24 h. C. Fluorescence and phase contrast (PH) analyses of HEK293T cells transfected with HA-Ev or HA-RTN3, followed by infection with VSV-eGFP (MOI = 0.05) at the indicated timepoints. Scale bar, 100 μm. D. The percentage of eGFP-positive cells in the same samples shown in (C) was analyzed by flow cytometry. E. Immunoblot analysis of HEK293T cells transfected with HA-Ev or HA-RTN3 together with plasmids encoding TBK1, NF-κB p65 and IRF3 (Flag-TBK1, Flag-p65 and Flag-IRF3) followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels analyzed by gray intensity scanning of blots (right). F. RT-PCR analysis of IFN- β, IFIT2, IFIT1, TNF- α, IL-6 and CCL20 mRNA levels in HEK293T cells transfected with HA-Ev or HA-RTN3 followed by infection with VSV-eGFP (MOI = 1) at the indicated timepoints. In (E), the data are representative of three independent experiments. In (A, B, F), the data are shown as the mean values ± SD (n = 3). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.

Article Snippet: The following antibodies were used in this study: anti-RTN3 (BA3533), purchased from BOSTER; anti-HA (M132-3), purchased from MBL; anti-Flag (AE005), anti-GFP (AE0122), were purchased from ABclonal; anti-β-actin (HC201-01), purchased from TransGen; anti-TRIM25 (67314-1-Ig), purchased from Proteintech; anti-GST (#2622), anti-Phospho-IKKα/β (#2697), anti-Phospho-TBK1 (#5483P), anti-TBK1 (#3504), anti-Phospho-IRF3 (#4947), anti-IRF3 (#4302), anti-Phospho-NF-κB p65 (#3033) and anti-NF-κB p65 (#8242) were purchased from Cell Signaling Technology; goat anti-mouse IRDye680RD (C90710-09) and goat anti-rabbit IRDye800CW (C80925-05), were purchased from Li-COR.

Techniques: Luciferase, Transfection, Plasmid Preparation, Activity Assay, Fluorescence, Infection, Flow Cytometry, Western Blot, Reverse Transcription Polymerase Chain Reaction

A. Immunoblot analysis of control HEK293T cells (HEK293T-shCtrl) and TRIM25 knockdown HEK293T cells (HEK293T-shTRIM25) transfected with HA-Ev or HA-RTN3 together with Flag-TBK1 and Flag-p65 and followed by VSV-eGFP infection (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels in (A) analyzed by gray intensity scanning of blots (right). B. CoIP and immunoblot analysis of HEK293T-shCtrl and HEK293T-shTRIM25 cells transfected with HA-Ub-K63 and increasing amounts of HA-RTN3 (+, ++) or HA-Ev together with GFP-RIG-I or GFP-Ev in the indicated groups for 24 h and followed by infection with VSV (MOI = 1) for 8 h. In (A, B), the data are representative of three independent experiments. In (A, left), the data are shown as the mean values ± SD (n = 6). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.

Journal: bioRxiv

Article Title: RTN3 inhibits RIGI-I-mediated antiviral responses by impairing TRIM25-mediated K63-linked polyubiquitination

doi: 10.1101/2020.12.01.407320

Figure Lengend Snippet: A. Immunoblot analysis of control HEK293T cells (HEK293T-shCtrl) and TRIM25 knockdown HEK293T cells (HEK293T-shTRIM25) transfected with HA-Ev or HA-RTN3 together with Flag-TBK1 and Flag-p65 and followed by VSV-eGFP infection (MOI = 1) at the indicated timepoints (left). Quantitative comparison of the indicated protein levels in (A) analyzed by gray intensity scanning of blots (right). B. CoIP and immunoblot analysis of HEK293T-shCtrl and HEK293T-shTRIM25 cells transfected with HA-Ub-K63 and increasing amounts of HA-RTN3 (+, ++) or HA-Ev together with GFP-RIG-I or GFP-Ev in the indicated groups for 24 h and followed by infection with VSV (MOI = 1) for 8 h. In (A, B), the data are representative of three independent experiments. In (A, left), the data are shown as the mean values ± SD (n = 6). *, p < 0.0332; **, p < 0.0021; ***, p < 0.0002; and ****, p < 0.0001 by Sidak’s multiple comparisons test.

Article Snippet: The following antibodies were used in this study: anti-RTN3 (BA3533), purchased from BOSTER; anti-HA (M132-3), purchased from MBL; anti-Flag (AE005), anti-GFP (AE0122), were purchased from ABclonal; anti-β-actin (HC201-01), purchased from TransGen; anti-TRIM25 (67314-1-Ig), purchased from Proteintech; anti-GST (#2622), anti-Phospho-IKKα/β (#2697), anti-Phospho-TBK1 (#5483P), anti-TBK1 (#3504), anti-Phospho-IRF3 (#4947), anti-IRF3 (#4302), anti-Phospho-NF-κB p65 (#3033) and anti-NF-κB p65 (#8242) were purchased from Cell Signaling Technology; goat anti-mouse IRDye680RD (C90710-09) and goat anti-rabbit IRDye800CW (C80925-05), were purchased from Li-COR.

Techniques: Western Blot, Transfection, Infection